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Author (up) Fu, T.; Touboul, D.; Della-Negra, S.; Houel, E.; Amusant, N.; Duplais, C.; Fisher, G.L.; Brunelle, A. url  doi
  Title Tandem Mass Spectrometry Imaging and in Situ Characterization of Bioactive Wood Metabolites in Amazonian Tree Species Sextonia rubra Type Journal Article
  Year 2018 Publication Analytical Chemistry Abbreviated Journal Anal. Chem.  
  Volume 90 Issue 12 Pages 7535-7543  
  Abstract Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this paper, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrynolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these γ-lactone compounds was achieved, providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray parenchyma cells but also in certain oil cells and, further, revealed the presence of previously unidentified γ-lactone, paving the way for future studies in biosynthesis.  
  Corporate Author Thesis  
  Publisher American Chemical Society Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0003-2700 ISBN Medium  
  Area Expedition Conference  
  Notes doi: 10.1021/acs.analchem.8b01157 Approved no  
  Call Number EcoFoG @ webmaster @ Serial 834  
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